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Best RNA-Seq aligner: A comparison of mapping tools
RNA-Seq has replaced microarrays for many applications in the area of biomarker discovery. The prices have been fallen substantially in recent years.... -
Script for Align some pattren and fetch highly similar sequence from gene
hello
I am new in programming, i want to match pattren in fasta sequence and extract sequence from fasta file
for example here is... -
aligning pre-assembled transcriptomes to genomes
Hi,
I have been given a pre-assembled transcriptome for my organism, which was generated using de novo assembly of RNAseq reads, assembly...Last edited by skeffington; 02-11-2015, 03:03 AM. -
clustal alignment
Hi all,
I have to plan a little my work, and I have a question I am not finding answer.
How long could a clustal run be?
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reads position in reference sequence(sam file)
Hi all,
there are some reads aligned. the .sam output file produced using bowtie, is like bellow and i want to extract aligned reads and... -
BowTie FP and FN - How to find ?
I am using BowTie 1 to find alignments of 150 bp reads. Can anybody help me how to find how much False Positive and False Negative it gave ? -
BWA -N options
I am using BWA tool to find alignment of reads:
Now, I use the command:
./bwa aln -n 2 -k 41 DB.fa SRR493081_1.fastq >... -
how to merge paired ends provided in separate files during (bfast) alignement
Hello,
I want to align illumina paired-end reads by using bfast. The point is that each end is provided in two separate .fastq files. ... -
which human Reference genome to use
I guess it is a very stupid question, but there is a difference between using the hg19 reference genome vs the GRCh37?
I want to align... -
Bfast alignement with paired end reads in separate files
Hello,
I am dealing with Illumina paired end reads (100 + 100) and I am trying to align them by using bfast. The point is that they are...
