Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • marco12345
    Member
    • Mar 2013
    • 19

    #1

    clustal alignment

    Hi all,

    I have to plan a little my work, and I have a question I am not finding answer.
    How long could a clustal run be?

    That would be using clustalw-2.1, and doing a multiple alignment on 10 fasta sequences of about 150 kb. The type of alignment is "slow/accurate", the ouput a PHYLIP file, and all the options are on default values.
    I'm thinking on running it on a MacBook 3.1 with 4 Gb of RAM.

    What do you think? Would that be too much for a laptop?

    Thanks in advance.

    Marco
  • chuanchao
    Junior Member
    • Aug 2013
    • 3

    #2
    why not use mega?

    Comment

    • rhinoceros
      Senior Member
      • Apr 2013
      • 372

      #3
      No, but I'd use muscle instead of clustal..
      savetherhino.org

      Comment

      • jimmybee
        Senior Member
        • Sep 2010
        • 119

        #4
        From my experience with mitochondrial and chloroplast alignments, Id say less than a week. You could try Clustal-omeg. Im pretty sure its multi-threaded

        Your laptop should handle it

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
          by SEQadmin2



          CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

          Despite this, “CRISPR helped turn genome editing from a specialized technique into
          ...
          07-31-2026, 11:01 AM
        • SEQadmin2
          Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
          by SEQadmin2


          Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

          The systematic characterization of the human proteome has
          ...
          07-20-2026, 11:48 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, 08-06-2026, 07:41 AM
        0 responses
        23 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 08-03-2026, 10:13 AM
        0 responses
        35 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 07-31-2026, 02:55 AM
        0 responses
        43 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 07-24-2026, 12:17 PM
        0 responses
        26 views
        0 reactions
        Last Post SEQadmin2  
        Working...