Hello,
I am making libraries for RADseq. I am trying out the Covaris ME220 for shearing the digested DNA. The shearing seems to be highly...
27 results in 0.0030 seconds.
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Shearing digested DNA for RADseq
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Agilent SureSelectXT - input
Hello,
I have been using the recommended input of 3ug for Aglient SureSelectXT but when I QC post covaris/post clean-up, I am left with...Last edited by sward; 10-21-2016, 03:24 AM. -
Covaris tubes
Has anyone tried an alternative tube type or plate other than the glass microTUBEs for shearing gDNA using the Covaris LE220 series?
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Covaris M220 service plan
Hi all,
Our department is considering purchasing a Covaris M220 so we can begin generating libraries in house. A one year service plan... -
Tapestation quality peaks show unwanted larger fragments.
Hi all,
I used Covaris to shear human genomic DNA at a set size of 300bp and 200bp respectively. This is to sequence my samples...Last edited by mzahir89; 08-19-2015, 02:20 AM. -
Covaris AFA Grade Water
Hello,
Is the "super special" water needed for a Covaris M220 just high quality ddH2O, or is it something else?
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genomic DNA shearing with Covaris 12*12 tubes
Hello, I am calibrating a new protocol and I need to shear my cross-linked DNA (2*10^7 RAW cell line) to an average size of 1000 bps. I'm using Covaris... -
covaris sonication for ChIRPseq
Hi,
I am planning to do ChIRPseq which requires sonication of chromatin down to around a 100-500bp range. The protocol recommends using... -
ChIP-exo: shearing bacterial genomic DNA using Covaris S220
Hello everyone,
I'm trying to optimize sonication for ChIP-exo for better reproducibility. I have never utilized the Covaris S220 system... -
Covaris sample volume
Is anyone else having problems pipetting samples into the Covaris microTUBES? I've been frequently seeing air bubbles (presumably the result of surface... -
Position-specific GC bias?
Hi, I've been having an unusual problem with my reads. I've been doing exome sequencing on a HiSeq 2000, but I'm getting only about 40% GC content at... -
High concentration Covaris Shearing
Hi all,
I'm currently using the Covaris S2 to fragment my DNA to approximately 1.5-2kb.
Mainly so I can ligate to perform an inverse... -
Shearing plant chromatin using Covaris S220
Does anyone have suggestions for shearing chromatin isolated from Arabidopsis leaf tissue (or any plant material, for that matter) using the Covaris S220.... -
Covaris M220 or S220?
Hi all!
Working in a lab about to install the HiSeq system.
We expect a throughput of about 48 samples a week.
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Covaris S2 Systems for sale
We are selling two Covaris S2 Systems for $15,000 each. Both of them have the degassing pump changed last year and so are practically brand new. Just...
