Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Covaris M220 or S220?

    Hi all!

    Working in a lab about to install the HiSeq system.

    We expect a throughput of about 48 samples a week.

    But we are stuggling what shearing unit to buy; the Covaris M220 or the Covaris S220?

    Anyone that have an opinion about this feel free to respond.

    erl

  • #2
    Unhelpful off topic commercial content deleted
    Last edited by ECO; 08-07-2012, 02:41 PM. Reason: Unhelpful off topic commercial content deleted

    Comment


    • #3
      Originally posted by dalib View Post
      Unhelpful off topic commerical content deleted
      Hey Dalib! This type of post isn't good internet etiquette, and isn't acceptable here. Either answer his question, or post commercial content in the Vendor Forum.

      Thanks!

      Comment


      • #4
        To try to be helpful, 48 all at once? Or staggered throughout the week?

        The big 96-well systems are a huge step up in investment for that low of throughput if it's sprinkled throughout the week. The single tube system could totally handle that much if so. But the 96-well system is _great_ for reproducibility and walk-away automation, and will definitely give you a lot of room to grow. We don't think twice about shearing full plates now...toss it in in the PM and it's waiting for you in the morning.

        Comment


        • #5
          Thanks a lot for the replies, but I am sure we would not need a 96 well systeme and we will not have an bioruptor.

          Both M- and S- series machine from covaris is single tube systemes, but the M-series is half the price of the S-series. So is the M-series just a cheap version of the S-series, but is it giving the same quality results?

          erlandsen

          Comment


          • #6
            Looks like the M220 needs pricier microtubes (40% higher price). They might allow higher recovery though.
            From what I gather from the Covaris site, the M machine needs no waiting time but perhaps operates at 20 degrees instead of seven degrees C.

            Comment


            • #7
              I just bought the M220 and I'm very impressed with it so far. The cheaper price is what attracted me to it since it would have been very hard for our lab to afford the S series. The other advantage is the smaller bench footprint. There is only the instrument and no other chillers to take up space. The computer also fits on top of the instrument.

              As far as I can tell, the M220 is just a smaller version of the S220. There is no chiller (you operate at 20 degrees) so there's no wait time. You use a whole lot less water as well. It's so extremely easy to set up and use compared to the S220 (and that one is pretty easy too). So far my results have been even more consistent and with tighter bands than when I used the S220. All in all, I'm very happy with it and would recommend it to any lab. I can't think of any reason to get the S220 over the M220 now that it's out.

              Comment

              Latest Articles

              Collapse

              • seqadmin
                Exploring the Dynamics of the Tumor Microenvironment
                by seqadmin




                The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...
                07-08-2024, 03:19 PM
              • seqadmin
                Exploring Human Diversity Through Large-Scale Omics
                by seqadmin


                In 2003, researchers from the Human Genome Project (HGP) announced the most comprehensive genome to date1. Although the genome wasn’t fully completed until nearly 20 years later2, numerous large-scale projects, such as the International HapMap Project and 1000 Genomes Project, continued the HGP's work, capturing extensive variation and genomic diversity within humans. Recently, newer initiatives have significantly increased in scale and expanded beyond genomics, offering a more detailed...
                06-25-2024, 06:43 AM

              ad_right_rmr

              Collapse

              News

              Collapse

              Topics Statistics Last Post
              Started by seqadmin, Yesterday, 06:53 AM
              0 responses
              12 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, 07-10-2024, 07:30 AM
              0 responses
              34 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, 07-03-2024, 09:45 AM
              0 responses
              204 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, 07-03-2024, 08:54 AM
              0 responses
              213 views
              0 likes
              Last Post seqadmin  
              Working...
              X