hi everyone
I have a problem with PE module Hiseq 2000
Basically the instrument read well the read 1 and the read index, but when arrive...
19 results in 0.0024 seconds.
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low fluorescence intensities read 2, HiSeq 2000
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Volume of Output data in Illumina HiSeq 2000
Hi all,
Does Anyone know volume of output data for metagenimics samples (such as gut sample) in Illumina HiSeq 2000?
I asked... -
Looking for opinions on stopping HiSeq 2000 service contract
Hello everyone,
We currently have an older HiSeq 2000 housed in our facility (V3 runs only) that isn't currently getting much use because... -
Library quantification of Small-RNAseq
Hi!
We are doing Small-RNAseq libraries since a little bit (using TruSeq Small RNA prep kit) and we have problem quantifying the libraries accurately.... -
Extreme 5' nucleotide bias in 2nd pair Illumina Hiseq reads
Hi Everyone,
I'm new to the forum and have a question regarding an odd pattern in a new transcriptome dataset of Illumina HiSeq 2000 (v1.9)... -
Inconstant Cluster Density on HiSeq 2000
Since a couple of months our HiSeq 2000 shows inconstant Cluster Density.
We started having troubles when some library pools (we multiplex our libraries...Last edited by Miaranne; 01-14-2014, 02:42 PM. -
ChIP-Seq multiplexing
Hi all,
I have prepared a ChIP-Seq library with Illumina's ChIP-Seq DNA sample prep kit (IP-102-1001). It is in pretty good shape so I... -
HiSeq 2000 50PE or 100PE?
in my mind i think HiSeq 2000 100PE is better than 50PE, but there are some other teachers suggest me to do 50PE. they think 50PE' result is more accurate... -
Upgraded Illumina HiSeq Chemistry
I recently heard a rumor that Illumina is about to introduce a new chemistry that will allow for 2x300bp output. I can't seem to find any more information... -
Multiplex scheme for Hiseq run
Hi,
I have an issue I need to resolve regarding multiplexing libraries for a Hiseq run.
There are 96 libraries, I am making... -
Adventures in cluster density titration.
We have perennial issues with obtaining sane/consistent library concentrations prior to clustering. Those of you with a HiSeq will probably have this... -
Looking for high coverage dataset
Hi everyone,
I've been reading and learning for some time from this forum, it assisted me in solving some alignment problems!
... -
TSS plot. Drop of the signal at TSS.
Hi all,
I'm fairly new to NGS data analysis. My question is related to the shape of the coverage plots relative to TSS of ChIP against... -
Estimate a transcriptome
Hi all,
does anybody know how Can I estimate the transcriptome size if I don´t know the right size of a genome?
I would like to perform... -
CASAVA 1.8.2 configureBltoFastq error
Hello to everyone from a new user -I am trying to learn Casava 1.8.2. I am working with a single read 50 bp read and 7bp index run(6 base index--some...
