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  • HiSeq 2000 50PE or 100PE?

    in my mind i think HiSeq 2000 100PE is better than 50PE, but there are some other teachers suggest me to do 50PE. they think 50PE' result is more accurate when do the same data. Are they right? thank you!

  • #2
    Hi Lucky

    The maximum read length in hiseq is 100 there won't be much deference in data quality because after 75 cycles the laser power will be boosted so does intensities. It depends on for what application you want sequences.

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    • #3
      Originally posted by share View Post
      Hi Lucky

      The maximum read length in hiseq is 100 there won't be much deference in data quality because after 75 cycles the laser power will be boosted so does intensities. It depends on for what application you want sequences.
      thank you for your reply. I know that 50PE can be used in small RNA sequencing. then could you tell me some other items maybe use 50PE is better? very thank you!

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