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  • Pablin
    Junior Member
    • Apr 2011
    • 1
    #1

    Estimate a transcriptome

    Hi all,
    does anybody know how Can I estimate the transcriptome size if I don´t know the right size of a genome?
    I would like to perform multiplexing with more than 2 samples on the same lane on a HiSeq but don´t know how many more can be done...
    Thank you.

    Pablo
    Tags: hiseq 2000, size, transcriptome
    • westerman
      Rick Westerman
      • Jun 2008
      • 1104
      #2
      The transcriptome size has very little to do with the genome size (at least for eukaryotes). Consider that, for plants, Arabidopsis has a genome size of 157MB while Paris Japonica, supposedly the largest plant genome, has a genome size one thousand times that at ~150GB. Does PJ have a 1000 times as many transcripts? It is highly doubtful. :-)

      As for an estimate of your transcriptome size, take the transcriptome of a closely related organism and use that.

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