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Illumina Sample sheet ddRAD
I have produced a ddRAD library following Poland et al. 2012 . Can anyone help me set up the sample sheet for a mi-seq? I only have one PCR product but... -
Stacks process_radtags N in overhang
I have RADseq reads (using PSTI) where the first position in the overhang is almost invariably N. When I try to use process_radtags, it discards all of... -
help! RAD, Stacks process_radtags.pl script, ambiguous RADs
I produced a single digest RAD library using Sbf-I, a rare cutter, following a variation of the Etter (2011) protocol available on Michael Miller's website... -
Restriction-site Associated (RAD) services
Does anyone know of any other companies than Floragenex that perform RAD-tag library production and genotyping? I'm working on sole source justification.... -
Are RAD data strand-specific?
I think RAD data are not strand-specific, we can sequence both the two strand of DNA, but I found ustacks do regard one sequence and it's reverse complementary... -
Why RAD reads depth vary extremely？
I have got a RAD data set and assessed it. It seems the depth of RAD fragment vary differently, extremely different.
the reads(extract... -
How many individuals per population?
G'day Everyone,
I wasn't sure where to post this question; my apologies if I'm in the wrong spot.
I recently read this paper... -
RAD-seq SNPs: pooled population library prep?
G'day Everyone,
I am new to genomics and hope that I don't annoy you by asking a basic question.
I am thinking of using RAD-seq... -
How to Estimate the Average Coverage per RAD Locus
Hi all,
I am trying to estimate the sequencing effort needed for a valid SNP call using RADtags on a non-model organism.
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