Hi All,
We are trying to follow-up on some small RNA-sequencing that we have done. We would like to do some northern blots on the RNA and probe...
35 results in 0.0032 seconds.
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RNA size selection
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Size selected library is not amplified
Hi all,
I'm trying to make RNA library using "NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina"
After... -
Low efficiency in bead-based size selection. Can't get rid of large fragments.
Hi everyone!
We’re performing a bead-based size selection of double digested genomic DNA, targeting fragments between 400bp and 700bp,... -
PCR after size selection of cDNA library
Hi all,
Recently I'm trying to make cDNA library using Tag-seq protocol.
After amplifying my sample with illumina adapter, I select... -
Multiplexing BluePippin size selection
First post, but a longtime lurker!
Currently doing Illumina library preps for MiSeq sequencing, and even though we have mostly used TruSeq... -
Problematic ddRAD library post-size selection bioanalyzer trace
Hi everyone,
I'm preparing a ddRAD-seq plate for Illumina HiSeq 4000 sequencing and I'd like to know if anyone has seen similar Bioanalyzer... -
maximum size of fragment as input for BluePippin?
Hello all,
I ran a library on the BluePippin using a 2% agarose cassette (V1 marker), with the hope to size select from 150 to 450bp.... -
bluepippin size selection for SMRTbells
Hello,
at which step of the 10kb protocol for preparation of SMRTbell libraries do you run BluePippin 20kb cassette for size selection?... -
Cheap size fractionation for nanopore sequencing?
Hello,
I'm doing some 2D nanopore sequencing and I'm a bit disappointed with the number and cost of third-party products recommended in... -
E-gel vs BluePippin for narrow size selection???
Hi Everyone,
I am facing a problem with size selection of Covaris fragmented DNA. I would require insert sizes in the narrow range 700-800... -
Pippin-Prep - External or Internal markers?
Hi Seqanswers community,
I going to test size selection step in Pippin-Prep and I´m not sure about the advantages and disadvantages to... -
Pippin Elute directly into MiSeq Library
Hello, I recently got a PippinHT loaned to me for a demo, and I am using it for final library pool size selection before loading my library onto a MiSEQ.... -
Pooling Prior to Size-Selection
Greetings everyone,
I am a graduate student making his first foray into NGS. Briefly, I am employing a ddRAD protocol to generate my first... -
Make your own AMPure XP, cheaply
Like a lot of labs, we've replaced spin/vacuum columns with magnetic bead extraction for every part of our library construction protocols. However, the...Last edited by jwfoley; 11-01-2017, 09:14 AM. -
Sophie's choice: Random mate-pairs or gel selection in Illumina?
Hi guys!
I'm sequencing and assembling a de novo invertebrate genome of about 0,8Gb. I want to make a lane run of Illumina nextera mate-pairs,...Last edited by Marcela Uliano; 01-08-2015, 07:35 AM.
