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Hi All,
We are trying to follow-up on some small RNA-sequencing that we have done. We would like to do some northern blots on the RNA and probe for some short RNA molecules that we found (non miRNAs). The typical short RNA-seq library prep protocol does a size section after the adapters have been added and after converted to cDNA. Here, we would like to do a size selection on total RNA first, then do a northern blot. Also, we would need to use a substantially larger amount of RNA than you would for a sequence library. So, essentially, does anyone have a protocol to take a large amount of RNA (>10ug) and size select it for only those molecules <70nts long?
Thanks
We are trying to follow-up on some small RNA-sequencing that we have done. We would like to do some northern blots on the RNA and probe for some short RNA molecules that we found (non miRNAs). The typical short RNA-seq library prep protocol does a size section after the adapters have been added and after converted to cDNA. Here, we would like to do a size selection on total RNA first, then do a northern blot. Also, we would need to use a substantially larger amount of RNA than you would for a sequence library. So, essentially, does anyone have a protocol to take a large amount of RNA (>10ug) and size select it for only those molecules <70nts long?
Thanks