Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches to targeted...
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Targeted Sequencing: Choosing Between Hybridization Capture and Amplicon Sequencing
Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches to targeted...Last edited by seqadmin; 12-15-2025, 10:24 AM. -
Confusing Picard HsMetrics ON_BAIT_VS_SELECTED for near=250 and near=0
Hi, for an assay developed by my vendor, I ran latest Picard HsMetrics (2.21.8) with two different options for the same set of BAM files: one with a NEAR_DISTANCE=250... -
Concatenate fasta files and create partition file too?
Hello,
I'm concatenating ~1000 gene sequences and I'd like the final sequence file to also have a corresponding partition file that lists... -
Custom Panels for Targeted Sequencing
We offer MYbaits® as a complete system for efficient, in-solution target enrichment for high-throughput sequencing. The MYbaits family of products provides... -
Pooling Recommendations for SureSelect Target Capture
Bioo Scientific’s latest Tech Tip, Pooling Recommendations for SureSelect Target Capture, offers guidelines to help determine which option is best for... -
High capacity, low cost, targeted NGS
Hello SEQanswers
My name is Kurt Lamour and I run a company (Floodlight Genomics) that provides a new low cost option for targeted genetic sequencing... -
Probe designing tips for targeted NGS
Hello. I'm trying to analyse DNA methylation patterns of selected DMRs by the HiSeq system.
I'm planning to use biotin labeled DNA probes to pull... -
Frozen Dynabeads
My DNA-bait hybridization reaction appears to be failing. The Dynabeads I am using were frozen once in their buffer solution upon delivery. Does this...Last edited by dwhite31; 07-02-2015, 01:22 PM. -
Single Nucleotide Repeat Help Needed
I am trying to find the "true" genotype of several loci from target capture data. These are single nucleotide repeats between 15-18 bps long.... -
TruSeq Nano and hybridization?
I am interested in using the newer truseq nano kit for library preparation, but I am wondering if the products produced using the kit are suitable for... -
isses about gaps in the probes from NimbleDesign
We aim to use Nimblegen seqcap EZ choice library to target-sequence ~ 100 genes of interests including promoters, coding and intronic regions. NimbleDesign... -
NEXTflex™ Pre-Capture Combo Kit Simplifies Sequence Target Capture Library Prep
The NEXTflex™ Pre-Capture Combo Kit is the first complete kit designed to work in combination with the Roche Nimblegen v3 SeqCap EZ Exome and EZ Choice...
