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Error with per tile sequence quality (by FastQC)
**NEW beginner**
I have run some samples on Miseq. After a quality check by FastQC, some of my samples had error with per tile sequence quality at a specific position. If I am not wrong, I think it was caused by the preparation of flowcell becase the samples with this kind of error were shown at the same specific position.
My question is...
1.Please give me some explanations; what is the cause of this error?
2.How to solve this problem or how to improve the quality of my sequences?
3.Can I ignore this error?
4.I have heard that FilterByTile might can help me on this, but I do not know how to use it. Please give me some suggestions
Thank you in advance.
I have run some samples on Miseq. After a quality check by FastQC, some of my samples had error with per tile sequence quality at a specific position. If I am not wrong, I think it was caused by the preparation of flowcell becase the samples with this kind of error were shown at the same specific position.
My question is...
1.Please give me some explanations; what is the cause of this error?
2.How to solve this problem or how to improve the quality of my sequences?
3.Can I ignore this error?
4.I have heard that FilterByTile might can help me on this, but I do not know how to use it. Please give me some suggestions
Thank you in advance.