Hi,
Apologies for the newbie question but I find the concept of using paired end reads for de novo assembly somewhat confusing. From what I understand, the paired end reads are produced using a reference genome - is that correct?
But how can you pretend that you are doing a de novo assembly (e.g. agnostic of a reference) while utilising the paired end information? For me there seems to be a contradiction.
Thank you in advance for any responses!
--FB.
Apologies for the newbie question but I find the concept of using paired end reads for de novo assembly somewhat confusing. From what I understand, the paired end reads are produced using a reference genome - is that correct?
But how can you pretend that you are doing a de novo assembly (e.g. agnostic of a reference) while utilising the paired end information? For me there seems to be a contradiction.
Thank you in advance for any responses!
--FB.
Comment