Coverage required for Sanger based SNP detection and Genotyping

[gavin.oliver]

Does anyone know of an established acceptable figure in terms of times coverage required for SNP/calling and genotyping with Sanger sequences?

I know 20x is generally recommended for NGS but this is obviously a different scenario.

[sciencewu]

many papers using the Sanger sequences ,but didn't give a criteria . maybe 10x is enough.

[prm36]

We take 2x coverage for SNP calling in our lab.

Ventner's genome was done to 7.5x (http://www.nature.com/nrg/journal/v1...df/nrg2626.pdf)

Cheers

Paul

[sciencewu]

Did you get a good result ? what's FP and FN ?