to complete or not to complete

[masterpiece]

Hi guys,

I want to get opinion from you all. For some of us might think completing a genome assembly (in this case sample from microbe) might be easy. But for some of us with limited of experience and fund, this practice could be difficult and time consuming.

For example, fishing-out genes for drug target, is it important to get genome complete assembled or is it enough by working on partially complete genome assembly. When i said partial complete, means its on the scaffold stage.

I hope someone can enlighten me, on what situation complete genome should be a choice, and on what situation working with partially complete is enough.

thanks in advance

-kamal

[chkuo]

This is a rather complex question. One simple way to look at it is that a partial genome can tells you some of the genes that are present in the genome, however, if you want to know which genes are truly absent then you'd need a complete genome.