Hi,
I am planning to have a couple of BAC clones sequenced on a 454 and it looks like I have a choice between standard chemistry with a paired-end library, or titanium chemistry with no paired-ends. Does anyone have any experience with which option is likely to provide the better assembly? Does the longer read length with titanium chemistry out-weigh the disadvantage of not using paired-ends? The BAC clones are likely to have some regions of repetitive sequences, and they are from a reptile with no close relatives so there is no reference.
-Hilary
I am planning to have a couple of BAC clones sequenced on a 454 and it looks like I have a choice between standard chemistry with a paired-end library, or titanium chemistry with no paired-ends. Does anyone have any experience with which option is likely to provide the better assembly? Does the longer read length with titanium chemistry out-weigh the disadvantage of not using paired-ends? The BAC clones are likely to have some regions of repetitive sequences, and they are from a reptile with no close relatives so there is no reference.
-Hilary
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