We are preparing to do paired-end de novo sequencing on our GAIIx with the new kits. Is there a reason to run less than the maximum 100+ cycles on each end? There was a suggestion that the higher error rates at 100+ may make <100 cycles more desirable. My thoughts are more is better, particularly b/c a majority of the cost is linked to cluster generation.
-
-
This is usually true but if the more you produce is meaningless it is only a waste. For example one year ago we produce with the kit of 36 bases experiments of 45 bases with the result that we where oblige to throw away all the bases after the 36 ....Originally posted by bkingham View Post
If the kit is able to produce 100 bases that you have to produce only 100 and be happy if all the 100 bases are of good quality and can be used
Francesco
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment