Hi everyone, i have 4,46 Gigas of information on various sequencing of transcripts in various tissues of Illumina Miseq paired-end reads. I had assembly all these reads and i found that the mean deep of coverage is of 27,9X (Deep of coverage = efficiency of sequencing / efficiency of assembly)
My question here is, what is de minimun of the deep of coverage for obtain robust information of the assembled transcriptome in a de novo transcriptome analysis?
Thanks!
Best regards!
My question here is, what is de minimun of the deep of coverage for obtain robust information of the assembled transcriptome in a de novo transcriptome analysis?
Thanks!
Best regards!
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