Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Pipeline suggestions for gene detection

    Hi everybody!

    I've been searching this question through the internet and forum but I didn't find an answer.

    I'm working with a non annotated (Scaffold stage) genome (Leptinotarsa decemlineata or Colorado Potato Beetle). I want to know if a receptor from a close specie (Tribolium castaneum) wich is annotated and thus, available in FASTA, is within Colorado Potato Beetle genome. I want to know this because, in that case, I would want to silence it using mRNAi.

    So I think the first step is to run a BLAST, wich I already ran, and find if there is homology, wich I found in two scaffolds.

    From here, and sorry for bother you with my naive questions, I do not know what to do to finally get a presumptive mRNA(CDS) and/or information about introns and exons.

    My actual question is: Can you suggest me any pipeline using the appropriate bioinformatic tools to get that mRNA?

    Thank you in advance!
    To do is to be (Nietzsche) - To be is to do (Kant) - Do be do be do (Frank Sinatra)

Latest Articles

Collapse

  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    Today, 07:48 AM
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 07:17 AM
0 responses
11 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-02-2024, 08:06 AM
0 responses
19 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-30-2024, 12:17 PM
0 responses
20 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-29-2024, 10:49 AM
0 responses
28 views
0 likes
Last Post seqadmin  
Working...
X