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  • miRNA Discovery

    I may be a bit naive, but I'm trying to see if I can identify miRNA-precursors in a new genome assembly I recently made.

    I have available to me:
    • de novo assembled genome
    • 1x100bp Illumina total RNA RNAseq
    • Illumina sRNA RNAseq


    Is there any program that can whittle down potential miRNA-precursor loci using all three sets of sequences rather than going through everything by hand?

    I suppose in my head I see something like:
    map 100bp read to genome --> look for sRNAs that bind within the mapped 100bp potential precursor --> analyze the region to see if it looks decent to actually be a miRNA precursor --> add it to my list to go back and verify experimentally someday.

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