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  • genome de novo assembly costs

    Hello,

    I would like to get just a rough idea (to avoid the hassle of asking for quotes) of what is currently (Feb 2015) the price of a genome de novo assembly for a genome of around 0.5 pg or 500 Mb.

    How does it compare with a de novo transcriptome assembly in price?

    Thanks very much.
    Last edited by cip; 02-19-2015, 09:38 AM.

  • #2
    That depends what you need - for a reasonable assembly you need two high quality mate pair libraries, which can be pricey. Two channels of HiSeq with a shotgun library and two different mate pair libraries and the bioinformatics would cost you about 10 000 €. You can do a de novo transcriptome (wet lab normalized cDNA library) with bioinformatics for about half of that.

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    • #3
      Thanks a lot sarvidsson. I thought it would be in the scale of 100K, rather than 10K. How much is the bioinformatics to do the assembly?.

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      • #4
        PM sent.....

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        • #5
          Depending on the nature of your organism (e.g. heterozygosity) and your access to a PacBio RS you could get the sequences for a fantastic genome assembly for ~ $13k with PacBio reads.

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