Hello,
I was wondering if anyone had enough experience with Minimus2 to tell me what its default handling of Ns was. I am attempting to combine two fasta denovo assemblies, where one or both contain long stretches of Ns as a scaffold. The concern I have is whether minimus2 is replacing Ns with sequence if there is a match that stretches from outside the N region into the N gap.
Thoughts?
I was wondering if anyone had enough experience with Minimus2 to tell me what its default handling of Ns was. I am attempting to combine two fasta denovo assemblies, where one or both contain long stretches of Ns as a scaffold. The concern I have is whether minimus2 is replacing Ns with sequence if there is a match that stretches from outside the N region into the N gap.
Thoughts?
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