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genome assembly polisher that can use Sanger reads?

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  • genome assembly polisher that can use Sanger reads?

    We have a PacBio final assembly that requires some polishing to fix e.g. erroneous stop codons. No Illumina reads at hand but we do have a set of high quality Sanger reads from an older version of the genome. Is there a tool like Racon but can use Sanger reads to correct small errors in an assembly?

  • #2
    You could try to modify one of these error correction algorithms: http://wgs-assembler.sourceforge.net...index.php/PBcR

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