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  • chronix
    Junior Member
    • Mar 2011
    • 2

    #1

    how to remove N (gap) in sequences?

    I need help. How to remove N between in sequences using by velvet?
  • Rachel
    Junior Member
    • Dec 2008
    • 9

    #2
    Hi i am about to ask the same questions. I had some the NNNN between my sequences and I had done some PCR to close the gaps. In this case do anyone know what type of software to remove the NNNN and replce it with new sequences?

    Thanks

    Comment

    • Peter Bjarke Olsen
      Junior Member
      • Jan 2009
      • 8

      #3
      You can avoid the N's in the velvet output by using the -scaffold option when running velvetg.

      Best regards,

      Peter

      Originally posted by chronix View Post
      I need help. How to remove N between in sequences using by velvet?

      Comment

      • Peter Bjarke Olsen
        Junior Member
        • Jan 2009
        • 8

        #4
        One solution could be to use the obtained sequence together with velvet using the -long option.

        Originally posted by Rachel View Post
        Hi i am about to ask the same questions. I had some the NNNN between my sequences and I had done some PCR to close the gaps. In this case do anyone know what type of software to remove the NNNN and replce it with new sequences?

        Thanks

        Comment

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