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Hi all,
I am trying to set up a global methylation project using Agilent's Methyl-Seq system but I'm having some trouble analyzing my sequencing results.
I was hoping those out there who are also attempting Methyl-Seq could give me their opinion on a few steps that I feel could be the problem:
How many samples to multiplex? I am currently trying 4.
How much PhiX DNA to spike in to account for the sequence bias? I have been using 1%.
Any help would be greatly appreciated,
Marie
I am trying to set up a global methylation project using Agilent's Methyl-Seq system but I'm having some trouble analyzing my sequencing results.
I was hoping those out there who are also attempting Methyl-Seq could give me their opinion on a few steps that I feel could be the problem:
How many samples to multiplex? I am currently trying 4.
How much PhiX DNA to spike in to account for the sequence bias? I have been using 1%.
Any help would be greatly appreciated,
Marie