I have seen it posted a couple of times that SDS must be diluted to 0.1% or less prior to ChIP. Why is that and is there a way to get rid of it other than dilution? Thx!
-
-
Hi,
That is true since most antibodies used for IP are sensitive to high SDS concentration. You can do one of three things:
1. prepare nuclei, and shear in a non-ionic detergent shearing buffer
2. Titrate the SDS content of your shearing buffer to see how low you can go without effecting the shearing.
3. Dialyze your sample after shearing to get rid of SDS prior to IP.
Thank you
Hamid -
Thanks--I may try those! Although, do you think it would be bad to dilute my sample at least 1:10 to get SDS below 0.1%.Comment
-
Not if you remain within the IP volume of the protocol you are following. Everyone using 1% SDS buffer for shearing, dilutes in the ChIP buffer for the IP step anyways.
HamidComment
-
CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM -
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM
Comment