Tweet
I have seen it posted a couple of times that SDS must be diluted to 0.1% or less prior to ChIP. Why is that and is there a way to get rid of it other than dilution? Thx!
-
-
Hi,
That is true since most antibodies used for IP are sensitive to high SDS concentration. You can do one of three things:
1. prepare nuclei, and shear in a non-ionic detergent shearing buffer
2. Titrate the SDS content of your shearing buffer to see how low you can go without effecting the shearing.
3. Dialyze your sample after shearing to get rid of SDS prior to IP.
Thank you
Hamid -
Thanks--I may try those! Although, do you think it would be bad to dilute my sample at least 1:10 to get SDS below 0.1%.Comment
-
Not if you remain within the IP volume of the protocol you are following. Everyone using 1% SDS buffer for shearing, dilutes in the ChIP buffer for the IP step anyways.
HamidComment
-
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
Comment