expected mutation rates?

[adaptivegenome]

Is anyone aware of NGS-based estimates of mutation rates for mammalian cell culture? I know that NGS was used to estimate germline mutation rate in humans but I am interested in the mutation rate of human cells in culture. So this would be somatic/mitotic mutation rate.

[marcowanger]

I have no idea on studies using NGS, but some independent OLD papers suggests in vitro cell culture increase mutation rate by 10x

in vivo retionblastoma: 10x-8 order
(http://www.ncbi.nlm.nih.gov/pmc/arti...00396-0056.pdf)

in vivo Y chromosome
in vitro germlien: 10x-8, approaching 10-7
(http://www.pnas.org/content/89/15/7036.full.pdf)

Normal vs Malignant lymphocyte, 10x in malignant
(http://cancerres.aacrjournals.org/co...2/407.full.pdf)

Somatic mutation: 10-7
(http://cancerres.aacrjournals.org/co.../18/8111.short)

[adaptivegenome]

I have no idea on studies using NGS, but some independent OLD papers suggests in vitro cell culture increase mutation rate by 10x

in vivo retionblastoma: 10x-8 order
(http://www.ncbi.nlm.nih.gov/pmc/arti...00396-0056.pdf)

in vivo Y chromosome
in vitro germlien: 10x-8, approaching 10-7
(http://www.pnas.org/content/89/15/7036.full.pdf)

Normal vs Malignant lymphocyte, 10x in malignant
(http://cancerres.aacrjournals.org/co...2/407.full.pdf)

Somatic mutation: 10-7
(http://cancerres.aacrjournals.org/co.../18/8111.short)

There is a recent nextgen study that puts human y-chromosome mutation rate at 10^-8 but I too have not found a NGS-derived cell culture mutation rate. So I suppose this does put cell culture at 10X the mutation rate of the human germline.

Thanks.

[marcowanger]

There is a recent nextgen study that puts human y-chromosome mutation rate at 10^-8 but I too have not found a NGS-derived cell culture mutation rate. So I suppose this does put cell culture at 10X the mutation rate of the human germline.

Thanks.

Yes, I also found that paper, I remember it's in CELL press / Curr Bio. Anyway, I could not find any NGS study on somatic vs germline mutation rate too.