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  • dan
    wiki wiki
    • Jul 2008
    • 194

    #1

    In a 'typica' human exome sequencing experiment...?

    If I go for a 'typical', off-the-shelf human exome sequencing experiment from Illumina, what kind of sequencing is done? i.e. Single end or paired end? If the latter, what is the 'typical' insert size used?

    Cheers,
    Dan.
    Homepage: Dan Bolser
    MetaBase the database of biological databases.
  • adaptivegenome
    Super Moderator
    • Nov 2009
    • 436

    #2
    Originally posted by dan View Post
    If I go for a 'typical', off-the-shelf human exome sequencing experiment from Illumina, what kind of sequencing is done? i.e. Single end or paired end? If the latter, what is the 'typical' insert size used?

    Cheers,
    Dan.
    paired end; ~250 base insert size

    Comment

    • dan
      wiki wiki
      • Jul 2008
      • 194

      #3
      Thanks dude. Is there a reference for this, or you just know from experience?
      Homepage: Dan Bolser
      MetaBase the database of biological databases.

      Comment

      • adaptivegenome
        Super Moderator
        • Nov 2009
        • 436

        #4
        Originally posted by dan View Post
        Thanks dude. Is there a reference for this, or you just know from experience?
        I was quoting from experience but I just did a google search and found this:

        http://www.illumina.com/documents/pr...chment_kit.pdf

        There they say insert size of 180-280. So what I told you fits in that range.

        Comment

        • dan
          wiki wiki
          • Jul 2008
          • 194

          #5
          Awesome, thanks dude.
          Homepage: Dan Bolser
          MetaBase the database of biological databases.

          Comment

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