In a 'typica' human exome sequencing experiment...?

[dan]

If I go for a 'typical', off-the-shelf human exome sequencing experiment from Illumina, what kind of sequencing is done? i.e. Single end or paired end? If the latter, what is the 'typical' insert size used?

Cheers,
Dan.

[adaptivegenome]

If I go for a 'typical', off-the-shelf human exome sequencing experiment from Illumina, what kind of sequencing is done? i.e. Single end or paired end? If the latter, what is the 'typical' insert size used?

Cheers,
Dan.

paired end; ~250 base insert size

[dan]

Thanks dude. Is there a reference for this, or you just know from experience?

[adaptivegenome]

Thanks dude. Is there a reference for this, or you just know from experience?

I was quoting from experience but I just did a google search and found this:

http://www.illumina.com/documents/pr...chment_kit.pdf

There they say insert size of 180-280. So what I told you fits in that range.

[dan]

Awesome, thanks dude.