Header Leaderboard Ad

Collapse

IGV Green and Red reads at same locus.

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • IGV Green and Red reads at same locus.

    Hello,
    I am viewing bam files in IGV and have a question. These bam files are generated from whole genome paired-end sequencing performed on human genomic DNA using Illumina instrument.

    Attached IGV screenshot shows bam files aligned to human reference genome, alignment viewed as "pairs" and alignment colored by "insert size and pair orientation". As far as I know, green colored reads are indicative of tandem duplication whereas red colored reads are indicative of a deletion. But I don't know what it means when both red and green reads are present at the same genomic location (as shown in the attached screenshot)? Can anyone please help me understand this? Thanks!
    Attached Files

Latest Articles

Collapse

  • seqadmin
    How RNA-Seq is Transforming Cancer Studies
    by seqadmin



    Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...
    09-07-2023, 11:15 PM
  • seqadmin
    Methods for Investigating the Transcriptome
    by seqadmin




    Ribonucleic acid (RNA) represents a range of diverse molecules that play a crucial role in many cellular processes. From serving as a protein template to regulating genes, the complex processes involving RNA make it a focal point of study for many scientists. This article will spotlight various methods scientists have developed to investigate different RNA subtypes and the broader transcriptome.

    Whole Transcriptome RNA-seq
    Whole transcriptome sequencing...
    08-31-2023, 11:07 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 09:05 AM
0 responses
12 views
0 likes
Last Post seqadmin  
Started by seqadmin, Yesterday, 06:18 AM
0 responses
10 views
0 likes
Last Post seqadmin  
Started by seqadmin, 09-20-2023, 09:17 AM
0 responses
12 views
0 likes
Last Post seqadmin  
Started by seqadmin, 09-19-2023, 09:23 AM
0 responses
26 views
0 likes
Last Post seqadmin  
Working...
X