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Hi! I have samples sequenced with NextSeq for the experiment protocol setup. We tested different kits (Nextera, Kapa, Qiagen) and apart from the donor samples, we sequenced ATCC bacterial mix with the addition of human cells, as well as ATCC genomic DNA. What I observe in QC reports is first of all, per base sequence content bias, but also disturbing GC content distributions for ATCC samples (yellow ones are the bacterial mix, the red one is genomic DNA). Would that suggest contamination or some other issue, or could it be a characteristic of those samples?
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Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.
Somatic Genomics
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM