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MetaGenomics Analysis & Antibiotic resistance detection training in Hyderabad, India
WHAT: 1-Day Technical Hands-on Workshop in MetaGenomics Analysis in 16S rRNA & Antibiotic resistance detection
WHEN: 3 March 2020
WHERE: Hyderabad, India
Innovation & creativity is an integral part of the BDG Lifesciences and we always launch new programs on the newest topics. We have launched a 1-day Hands-on Workshop on MetaGenomics Analysis in 16S rRNA & Antibiotic resistance detection | Amplicon Sequencing & Nanopore Data Analysis in Hyderabad, Telangana State, India in which we will teach about the Metagenomics analysis of 16S rRNAand Nanopore data.
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PRACTICAL APPLICATION
EXPERIMENT 1 - on 16S rRNAdata where sequences were only the rRNA or ribosomal DNA of organisms generated through HTS (high throughput sequencing) reads to identify species in microbial or environmental DNA samples.
AIM OF THE EXPERIMENT- The aim of this experiment is to determine the micro-organisms from which the sequences in our sample are coming from, this is called taxonomic assignation. We try to assign sequences to taxons and then classify or extract the taxonomy in our sample.
EXPERIMENT 2- on Nanopore MinION sequencer data
AIM OF THE EXPERIMENT- The aim of this experiment is the reconstruction of full plasmid sequences and determining the presence of any antimicrobial resistance genes.
Certificate along with workshop material with tea/coffee and snacks will be provided.
WHEN: 3 March 2020
WHERE: Hyderabad, India
Innovation & creativity is an integral part of the BDG Lifesciences and we always launch new programs on the newest topics. We have launched a 1-day Hands-on Workshop on MetaGenomics Analysis in 16S rRNA & Antibiotic resistance detection | Amplicon Sequencing & Nanopore Data Analysis in Hyderabad, Telangana State, India in which we will teach about the Metagenomics analysis of 16S rRNAand Nanopore data.
Know more
PRACTICAL APPLICATION
EXPERIMENT 1 - on 16S rRNAdata where sequences were only the rRNA or ribosomal DNA of organisms generated through HTS (high throughput sequencing) reads to identify species in microbial or environmental DNA samples.
AIM OF THE EXPERIMENT- The aim of this experiment is to determine the micro-organisms from which the sequences in our sample are coming from, this is called taxonomic assignation. We try to assign sequences to taxons and then classify or extract the taxonomy in our sample.
- File Optimization- Loading of data & optimizing it according to our experiment.
- Quality Control- Check sequencing quality
- Sequence Alignment- Grouping/Alignment of reads
- Extraction of Taxonomic Information- Operational Taxonomic Units (OTUs)- Clustering Sequences
- Visualization- Examine diversity of species/taxa present within a single sample, Comparison of species/taxa diversity between two samples & Taxonomic Diversity- How many species are in a sample? What are the differences in species between samples?
EXPERIMENT 2- on Nanopore MinION sequencer data
AIM OF THE EXPERIMENT- The aim of this experiment is the reconstruction of full plasmid sequences and determining the presence of any antimicrobial resistance genes.
- Obtaining and preparing data
- Quality Control- NanoPlot to explore data
- De-novo Assembly
- Species and plasmids- Prediction of plasmid sequences and classes
- Antibiotic Resistance- Scan genome contigs for antimicrobial resistance genes
Certificate along with workshop material with tea/coffee and snacks will be provided.