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How to use ARB nifH database(Zehr et. al) for taxonomic assignment
Hello members,
I am new in metagenomics data analysis. Currently I am working on a nifH amplicon data. I have done quality filtering, merging and construction of ASV's using DADA2 denoising method in QIIME2. Now I have to do taxonomic assignment to the ASV's. A nifH gene sequence database is available by jzehr lab which is in .arb format.
I couldn't find out a way as to how can I use this database in .arb format to assign taxonomy.
Is there any specific tool or method to do this?
Is the sequence as well as its taxonomy data available in the arb database?
How databases in .arb format are viewed or used for such kind of data analysis?
Can anyone please guide me in this regards?
Thanks in advance.
I am new in metagenomics data analysis. Currently I am working on a nifH amplicon data. I have done quality filtering, merging and construction of ASV's using DADA2 denoising method in QIIME2. Now I have to do taxonomic assignment to the ASV's. A nifH gene sequence database is available by jzehr lab which is in .arb format.
I couldn't find out a way as to how can I use this database in .arb format to assign taxonomy.
Is there any specific tool or method to do this?
Is the sequence as well as its taxonomy data available in the arb database?
How databases in .arb format are viewed or used for such kind of data analysis?
Can anyone please guide me in this regards?
Thanks in advance.