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  • wishSEQ
    started a topic SOLiD RNA-Seq protocol ver 2 - no PCR products

    SOLiD RNA-Seq protocol ver 2 - no PCR products

    Hi,
    I just finished preparing some libraries for SOLiD RNA-Seq using the new protocol (The one which does not use the Ambion fractionator). After doing a Bioanalyzer run I see that there are no PCR products.

    Does anybody have experience with the new protocol? Does it need optimization ? Comments greatly appreciated.

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  • seqadmin
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