Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • mapardo
    Junior Member
    • Jun 2011
    • 8

    #1

    htseq count missing last mapped fragment

    I am using the program htseq-count v0.5.3 with an standard sam file from the mapping of a set of paired end reads. Comparing this file with the output file that can be obtained with the option '-o', I've realized that the mapping for the last fragment (two lines) is missing. Furthermore, I check the resulting count and one mapping is missing as well.

    Has anybody got the same results? Is there any fix to solve this?

    Thank you.
  • Simon Anders
    Senior Member
    • Feb 2010
    • 995

    #2
    Originally posted by mapardo View Post
    I am using the program htseq-count v0.5.3 with an standard sam file from the mapping of a set of paired end reads. Comparing this file with the output file that can be obtained with the option '-o', I've realized that the mapping for the last fragment (two lines) is missing. Furthermore, I check the resulting count and one mapping is missing as well.
    This is a bug, which I fixed a few months ago, in version 0.5.3p1. (Thanks go to Justin Powell who noticed the bug first and even sent me a patch.)

    Comment

    • mapardo
      Junior Member
      • Jun 2011
      • 8

      #3
      Sorry. I didnt realize that I was using a version that wasnt updated. Thank you both.

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM
      • SEQadmin2
        New Genomics Technologies Take Aim at Long-Standing Limits
        by SEQadmin2


        Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

        We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
        ...
        09-28-2026, 10:25 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 10-15-2026, 10:52 AM
      0 responses
      26 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-14-2026, 05:39 AM
      0 responses
      31 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-13-2026, 09:56 AM
      0 responses
      28 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-09-2026, 11:14 AM
      0 responses
      28 views
      0 reactions
      Last Post SEQadmin2  
      Working...