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  • papori
    Senior Member
    • Dec 2010
    • 181
    #1

    directional mRNA sequencing on amplified RNA

    Hi,
    Do anyone know on paper of a directional mRNA(strand specific) sequencing on amplified RNA?
    Thanks
    pap
    Tags: None
    • steven
      Senior Member
      • Aug 2009
      • 269
      #2
      Here is an interesting comparative study from Levin et al:
      Comprehensive comparative analysis of strand-specific RNA sequencing methods - PMC
      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3005310/
      Strand-specific, massively-parallel cDNA sequencing (RNA-Seq) is a powerful tool for novel transcript discovery, genome annotation, and expression profiling. Despite multiple published methods for strand-specific RNA-Seq, no consensus exists as to ...

        Comment

        • papori
          Senior Member
          • Dec 2010
          • 181
          #3
          Thanks steven,
          i am looking for paper that do amplification on low amount of RNA and then sequence it by strand specific..
          Do you know?

          i knew the paper that you mention, and its a really good one, but it isnt what i am looking for

            Comment

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