Hi,
we will sequence small RNAs. We have isolated both small RNAs and total RNA. Anyone know what sequencing technology to use? Is it possible to sequence RNA molecules directly? We would prefer not to have any PCR amplifications in the library prep.
Thanks,
Jon
-
The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...07-08-2024, 03:19 PM