2 questions. What are people using to isolate total RNA for the small RNA prep and has anyone found a way to multiplex small RNA samples?
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Hello,
We will prepare miRNA-Seq library and send it out for sequencing on a GAII. Can anyone confirm that these adapter/Primer sequences first reported by ECO have (can) been used successfully on the GAII (and presumably GAIIx)?
Small RNA oligonucleotide sequences
RT Primer
5' CAAGCAGAAGACGGCATACGA
5' RNA Adapter
5' GUUCAGAGUUCUACAGUCCGACGAUC
3' RNA Adapter
5' P-UCGUAUGCCGUCUUCUGCUUGUidT
Small RNA PCR Primer 1
5' CAAGCAGAAGACGGCATACGA
Small RNA PCR Primer 2
5' AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA
Small RNA Sequencing Primer
5' CGACAGGTTCAGAGTTCTACAGTCCGACGATC
Was (is) there a GAI instrument? Does it require different Adapter/Primer sequences? Can't find any evidence of a GAI version on Illumina website.
Thanks in advance
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