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  • miRNA mapping w/ Novoalign

    *Posted also under Bioinformatics

    Hi all,

    I am trying to map two mature miRNA sequences to the human genome. My background is not bioinformatics or comp-sci but, having searched the web, I am under the impression I need to use Novoalign or Bowtie.

    The problem is that when I get to these websites and read about how to use these programs, my eyes gloss over and I am like

    Reading the help guides has not added any clarity.

    Can anyone help a non-computer person understand how to use Novoalign? I am using a MacOSx. I am not sure if I need adapter stripping - all I know is that the miRNA I want to map are "mature" sequences from the literature.

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