*Posted also under Bioinformatics
Hi all,
I am trying to map two mature miRNA sequences to the human genome. My background is not bioinformatics or comp-sci but, having searched the web, I am under the impression I need to use Novoalign or Bowtie.
The problem is that when I get to these websites and read about how to use these programs, my eyes gloss over and I am like
Reading the help guides has not added any clarity.
Can anyone help a non-computer person understand how to use Novoalign? I am using a MacOSx. I am not sure if I need adapter stripping - all I know is that the miRNA I want to map are "mature" sequences from the literature.
-
The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...07-08-2024, 03:19 PM