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How would I go about building a GTF file from either the FASTA file or from this infromation
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I think in FASTA there is no enough information to create a GTF file, but genebank files can be converted by this one. -
Thank you very much! I'm starting the process now.Comment
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Running Python
Ok I can't seem to get the python program to run, I've moved the file over to our server and tried to run it using the command given -
"cat file.gb | gb2gtf.py > file.gtf"
by inserting my files location for file.gb and then putting the output for file.gtf. I still receive the error -
gb2gtf.py: command not found
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Had to include the directory for the program as well
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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