Fpkm

[gregman31]

I was wondering what does FPKM means in REAL English? "fragments per kilobase of exon per million fragments mapped" clearly means nothing to me... Is that a the number of times a certain sequence has been red? or something similar to that?

Also what is a fragment exactly? Is it a positive read? Does someone know of any website, book or else, that explain all these jargons?

Thanks

[dpryan]

Google is your friend. See also the Trapnell et al. paper from 2010.

[gregman31]

Thanks very much for your reply.