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  • Normalizing Targeted RNA-seq Data

    I'd like to perform a targeted RNA-seq experiment to not only identify splice variants but also measure gene expression levels of the normal and abnormal variants I capture.

    Does anyone have any suggestions or direct me to some publications that can provide some guidelines as to the number of housekeeping transcripts I should include in my capture design such that my bioinformatics group can provide me with expression levels of the targeted transcripts/novel splice variants I want to capture?

    From my qPCR experience I was thinking that at least 3 would be needed but figured I'd ask the broader community to see if there was any consensus.

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