Hi everyone,
I want to prepare several libraries for RNA-seq using the Illumina Hi-Seq.
I am considering several kit options for making said libraries:
Illumina's TruSeq Stranded Total RNA Kit [dUTP-based]
Illumina's Directional Protocol [adaptation of small RNA-seq protocol, ligation]
Epicentre's v2 RNA-Seq Library Preparation Kit [Tag/Block]
Which of these libraries would you recommend for higher yields, greater accuracy, cost-effectiveness, etc?
I would like to barcode each library individually, for which option 1 (Illumina TruSeq Stranded) seems like a good option, since the adapters are already supplied with 12 different indices.
Looking forward to hearing your opinions.
C
I want to prepare several libraries for RNA-seq using the Illumina Hi-Seq.
I am considering several kit options for making said libraries:
Illumina's TruSeq Stranded Total RNA Kit [dUTP-based]
Illumina's Directional Protocol [adaptation of small RNA-seq protocol, ligation]
Epicentre's v2 RNA-Seq Library Preparation Kit [Tag/Block]
Which of these libraries would you recommend for higher yields, greater accuracy, cost-effectiveness, etc?
I would like to barcode each library individually, for which option 1 (Illumina TruSeq Stranded) seems like a good option, since the adapters are already supplied with 12 different indices.
Looking forward to hearing your opinions.
C
