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  • bernardo_bello
    Member
    • May 2012
    • 49
    #1

    RT-qPCR: which dilutions in calibration curve?

    I've seen in MIQE guidelines that calibration curves a needed when performing RT-qPCR. I'll use genomic DNA to perform these calibration curves.

    My question is that I don't know the range of concentrations to test.

    The genes I will check for differential expression have logFC between 2-8 (base 2 log).

    50 ng/μL is the sample concentration I will have when performing the Comparative CT Experiment, but 98% is ribosomal RNA.

    Please let me know you suggestions and possible reference books/papers to revise.

    Thanks for you help. Best regards, Bernardo
    Last edited by bernardo_bello; 12-04-2013, 10:43 AM.
    Tags: qpcr, rna-seq
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