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Are these excluded from Tophat if they are from diffferent chromosomes?
Any suggestions on packages that can do this determination?
Any suggestions on packages that can do this determination?
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I donot think so....but there are some packages for DNA fusion/translocation detection.. -
townway, could you point to dna fusion packages? I know pemer is a good one for translocation detection, but probably not for rna-seq data--
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right now there are some for DNA, like pemer, breakdancer. and I have not seen one for rna-seq. if you know, please post me as wellComment
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I think for tophat, it does not report fusion transcript from different chromosomeComment
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Including TopHat there are many more tools which can detect fusions both within and between chromosomes.Comment
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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