Hi all,
I have download the mRNA and microRNA NGS data from TCGA for BRCA.
for downloading the microRNA data, I choosed the miRNASeq from filter setting.
Now I have the expression level of miRNA in diffrent samples.
The question for is that, which kind of microRNA they are quantified ? is it all mature miRNA ? or mirna precursor are also there ? They seqenced miRNA which they got from gel electrophoresis ?(to be sure that, all of them have same length in case of mature miRNA).
But when I look at the miRNA IDs; there is some problem:
for example : they have expression level for hsa-mir-135a-2 , which when I search for it in miRBase, it's stem loop and it's mature form in miRbase is hsa-miR-135a-5p . so now I'm really in trouble to undersrand that expression level of which type of miRNA are quantified ?
would someone clarify it more ?
I have download the mRNA and microRNA NGS data from TCGA for BRCA.
for downloading the microRNA data, I choosed the miRNASeq from filter setting.
Now I have the expression level of miRNA in diffrent samples.
The question for is that, which kind of microRNA they are quantified ? is it all mature miRNA ? or mirna precursor are also there ? They seqenced miRNA which they got from gel electrophoresis ?(to be sure that, all of them have same length in case of mature miRNA).
But when I look at the miRNA IDs; there is some problem:
for example : they have expression level for hsa-mir-135a-2 , which when I search for it in miRBase, it's stem loop and it's mature form in miRbase is hsa-miR-135a-5p . so now I'm really in trouble to undersrand that expression level of which type of miRNA are quantified ?
would someone clarify it more ?
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