Hi All,
I am noticing evidence of chimeric reads coming from the ion proton, ie, the reads are half my organism of interest (lets say human) and half something else (Half ERCC control).
When BLAST'ed to nt, half the read hits human, and half it hits an ERCC control fragment. This is not a one off, I see it in data generated in-house and also some public human RNAseq data that I downloaded.
Has anyone else seen this? Are you aware of any documentation on these chimers? Do you agree with my interpretation of the result?
The example read is from SRA:
>SRR902918.363 AF94H:00015:07753 length=142
CGCATAGTCGCCATTCACAGATTTGCTCGGCAATCAGTACTGAAAGGCTAAGGCAGGTGAATCACCTGAGCTCAGGAGTTCAAGACCA
Thanks for your help.
I am noticing evidence of chimeric reads coming from the ion proton, ie, the reads are half my organism of interest (lets say human) and half something else (Half ERCC control).
When BLAST'ed to nt, half the read hits human, and half it hits an ERCC control fragment. This is not a one off, I see it in data generated in-house and also some public human RNAseq data that I downloaded.
Has anyone else seen this? Are you aware of any documentation on these chimers? Do you agree with my interpretation of the result?
The example read is from SRA:
>SRR902918.363 AF94H:00015:07753 length=142
CGCATAGTCGCCATTCACAGATTTGCTCGGCAATCAGTACTGAAAGGCTAAGGCAGGTGAATCACCTGAGCTCAGGAGTTCAAGACCA
Thanks for your help.
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