TopHat multireads?

SEQanswers June Challenge Has Begun!

The competition has begun! We're giving away a $50 Amazon gift card to the member who answers the most questions on our site during the month. We want to encourage our community members to share their knowledge and help each other out by answering questions related to sequencing technologies, genomics, and bioinformatics. The competition is open to all members of the site, and the winner will be announced at the beginning of July. Best of luck!

For a list of the official rules, visit (https://www.seqanswers.com/forum/sit...wledge-and-win)

See less

gthomas

Junior Member

Join Date: Aug 2009

Posts: 3
- Share
- Tweet
#1

TopHat multireads?

06-04-2010, 09:30 AM

Hi,

I am playing with some RNA-seq data and am trying to understand how TopHat is reporting its multireads. Can anybody explain how the locations of multiple hits are reported in the SAM output please?

Cheers,
Graham
Tags: multireads, rna-seq, tophat

Previous template Next

A Review of Next-Generation Sequencing Methods for Studying Epigenetics—Part 2

by seqadmin

In the first segment of our review of epigenetic sequencing methods, we covered methylation detection, chromatin accessibility, and protein-DNA interactions. This final part of our review will break outside of the standard analysis and cover some influential multidimensional methods.

Spatial Epigenomics
“Epigenetic assays, whether conducted at the single-cell or bulk level, have played a crucial role in unraveling the underlying mechanisms of diseases and...
- Channel: Articles
Yesterday, 06:48 AM
A Review of Next-Generation Sequencing Methods for Studying Epigenetics—Part 1

by seqadmin

Developments in sequencing technologies and methodologies have transformed the field of epigenetics, giving researchers a better way to understand the complex world of gene regulation and heritable modifications. This article explores some of the diverse sequencing methods employed in the study of epigenetics, ranging from classic techniques to cutting-edge innovations while providing a brief overview of their processes, applications, and advances.

Methylation Detect...
- Channel: Articles
05-31-2023, 10:46 AM
Differential Expression and Data Visualization: Recommended Tools for Next-Level Sequencing Analysis

by seqadmin

After covering QC and alignment tools in the first segment and variant analysis and genome assembly in the second segment, we’re wrapping up with a discussion about tools for differential gene expression analysis and data visualization. In this article, we include recommendations from the following experts: Dr. Mark Ziemann, Senior Lecturer in Biotechnology and Bioinformatics, Deakin University; Dr. Medhat Mahmoud Postdoctoral Research Fellow at Baylor College of Medicine;...
- Channel: Articles
05-23-2023, 12:26 PM

Topics	Statistics	Last Post
Investigating the Genetics of the Human Vocal System by seqadmin Started by seqadmin, Yesterday, 10:20 AM	0 responses 8 views 0 likes	Last Post by seqadmin Yesterday, 10:20 AM
Analysis of West Nile Virus Explores Transmission Dynamics in Animal Species by seqadmin Started by seqadmin, 06-07-2023, 07:14 AM	0 responses 13 views 0 likes	Last Post by seqadmin 06-07-2023, 07:14 AM
BabySeq Project: Unveiling Medically Actionable Variants by seqadmin Started by seqadmin, 06-06-2023, 01:08 PM	0 responses 13 views 0 likes	Last Post by seqadmin 06-06-2023, 01:08 PM
Genetic Discoveries Transform Our Understanding of Primate Diversity and Behavior by seqadmin Started by seqadmin, 06-01-2023, 08:56 PM	0 responses 166 views 0 likes	Last Post by seqadmin 06-01-2023, 08:56 PM

Header Leaderboard Ad

Announcement

SEQanswers June Challenge Has Begun!

TopHat multireads?

Latest Articles

ad_right_rmr

News